identification of a cdna sequence coding for kruppellike factor 2b (klf2b) from the skin mucosa of common carp (cyprinus carpio)

kruppel-like factors (klfs) are a highly related zinc-finger family of transcription factors implicated inthe regulation of the eukaryotic cellular growth and differentiation of a diverse set of cells in mammal. usingrt-pcr technique, a 456 bp cdna fragment encoding n-terminus part of a klf2b was isolated from the skinmucosa of common carp (cyprinus carpio) using two degenerative oligonucleotide primers. use of thisfragment as a probe allowed the isolation of a larger cdna clone through the searching of the genbankexpressed sequence tag database. the size of the amplified product is 1157 bp, which encodes a polypeptide of 274 amino acid residues with a predicted molecular mass of 30.359 kda and theoretical pi of 4.88. the deduced amino acid sequence exhibited 79, 54, and 53% identity to the homologous klf2b identified from zebrafish danio rerio, spotted green pufferfish tetraodon nigroviridis and atlantic salmon salmo salar, respectively. the common carp protein is 50% similar to klf2 orthologues in african clawed frog xenopus laevis, 44% in chicken gallus gallus and is 30% similar to the mammalian klf in house mouse musmusculus.